Fig 1.

Immunization, sampling, and study design. Two priming immunizations with an equal mixture of the 3 separate Ad5hr recombinants listed were given to the experimental macaque groups at weeks 0 and 12. Groups were defined by the route of Ad immunizations, either intranasal at week 0/intratracheal at week 12 (i.n./IT) or SL, Ivag, or IR at both weeks 0 and 12. The total number of animals in each group is shown in parentheses. Control animals receiving empty vector plus Ad5hr-GFP priming were divided among all four route groups. Experimental macaques received SIV gp120 in MPL-SE intramuscularly (IM) at weeks 24 and 36. Controls received adjuvant only. Biopsy, necropsy, and immunogenicity time points are listed, with the total number of animals sampled indicated in parentheses. Tissues sampled for each time point are listed. Arrows, time of immunizations; solid lines, time of biopsies; heavy dashed lines, time of necropsies; long dashed lines, time of immunogenicity measurements; Mes LN, mesenteric lymph node; Ing LN, inguinal lymph node.