Table 1.
Vectors and strains used in this study
| Plasmid or strain | Relevant phenotype, genotype, or description | Source |
|---|---|---|
| Plasmids | ||
| pCR4-TOPO | Ampr Kanr; cloning vector for sequencing | Invitrogen |
| pRSET-A | Ampr; vector for gene expression | Invitrogen |
| pFR.1 | pRSET-A vector with ferredoxin and reductase genes | This study |
| pFRR.1 | pFR.1 containing α/β subunit genes of putative RHD-1 | This study |
| pFRR.2 | pFR.1 containing α/β subunit genes of putative RHD-2 | This study |
| pFRR.5 | pFR.1 containing α/β subunit genes of putative RHD-5 | This study |
| pFRR.6 | pFR.1 containing α/β subunit genes of putative RHD-6 | This study |
| pFRR.7 | pFR.1 containing α/β subunit genes of putative RHD-7 | This study |
| pFRR.8 | pFR.1 containing α/β subunit genes of putative RHD-8 | This study |
| Strains | ||
| Mach1-T1 | F− ϕ80lacZΔM15 ΔlacX74 hsdR (rK− mK+) ΔrecA1398 endA1 tonA | Invitrogen |
| BL21(DE3)pLysS | F−ompT hsdSB(rB− mB−) gal dcm (DE3) pLysS (Camr) | Invitrogen |
| Variovorax sp. strain LY1 | Isolated from simplified enrichment culture | This study |
| Pseudomonas sp. strain BW1 | Isolated from simplified enrichment culture | This study |