Table 1.
Antibiotic susceptibility of selected sphingomonads and effect of rpsL1
| Strain | Growth with each antibiotica |
||||
|---|---|---|---|---|---|
| Wild type |
+ pAK126a-rpsL1 |
||||
| Tc10 | Km50 | Ca50 | Sm200 | Sm50 and Tc10b | |
| Sphingomonas sp. Fr1 (DSM 24958) | − | − | + | + | − |
| Sphingomonas sp. C3 (DSM 24957) | − | − | + | + | − |
| Novosphingobium rosa (DSM 7285) | − | − | + | + | − |
| Sphingomonas mali (DSM 10565) | − | − | + | + | − |
| Sphingomonas aerolata (DSM 14746) | − | − | − | +c | − |
| Sphingobium indicum (DSM 16412) | − | − | + | +c | − |
| Sphingobium rhizovicinum (DSM 19845) | − | − | + | + | − |
| Sphingomonas roseiflava (CIP 106847) | − | − | + | + | − |
| Sphingomonas wittichii (DSM 6014) | − | − | + | + | − |
a
Growth of wild-type strains (wild type) or otherwise-wild-type strains expressing rpsL1 from pAK126a (+ pAK126a-rpsL1) was assessed on nutrient broth (NB) supplemented with different antibiotics. Tc10, 10 μg tetracycline/ml; Km50, 50 μg kanamycin/ml; Ca50, 50 μg carbenicillin/ml; Sm200, 200 μg streptomycin/ml. +, growth after 2 days of incubation; −, no growth after 5 days of incubation.
b
A total of 10 μg tetracycline/ml was added for maintenance of pAK126a-rpsL1.
c
Growth was observed at a concentration of 50 μg streptomycin/ml but not at 200 μg streptomycin/ml.