Fig 1.
HPLC-MS chromatograms of the toxic methanol extracts of P. tundrae E8a and B. cereus NS-58 (cereulide producing [5]) under the same HPLC conditions. (A) Total ion chromatogram (TIC) of the extract of P. tundrae E8a. Two peaks were found, peaks 1 and 2, that were toxic in the rapid sperm assay, with retention times of 13.7 min (peak 1) and 15.8 min (peak 2). (B) Mass spectrum of peak 1, named paenilide. (C) Mass spectrum of peak 2, named the paenilide homolog (homopaenilide). (D) Total ion chromatogram of the extract of B. cereus NS-58 containing cereulide (9.7 min) and the cereulide homolog (11.4 min). (E) Mass spectrum of cereulide. (F) Mass spectrum of the cereulide homolog.