. 2012 May;78(10):3539–3551. doi: 10.1128/AEM.07657-11

Table 3.

TDA, pigment, and homoserine lactone production of the strains DSM 17395, WP38 (pgaI mutant), and WP52 (pgaR mutant) grown on MB and mineral medium with different sole carbon sources

Substrate	Strain	OD₆₀₀^a	Activity or growth characteristic^b
Substrate	Strain	OD₆₀₀^a	TDA production	Pigmentation (OD₃₉₈)	HSL production^c
MB	DSM 17395	0.12	+	+	+
	WP38	0.12	−	−	−
	WP52	0.12	−	−	+
Peptone + yeast extract	DSM 17395	0.18	+	+	+
	WP38	0.11	−	−	−
	WP52	0.10	−	−	+
Casamino Acids	DSM 17395	0.15	+	+	+
	WP38	0.10	−	−	−
	WP52	0.10	−	−	+
Phenylalanine	DSM 17395	0.10	+	+	+
	WP38	0.11	+	+	−
	WP52	0.12	+	+	+
Phenylacetate	DSM 17395	0.05	+	+	+
	WP38	0.06	−	−	−
	WP52	0.05	−	−	+
Glutamate	DSM 17395	0.26	+	+	+
	WP38	0.23	−	−	−
	WP52	0.21	−	−	+
Pyruvate	DSM 17395	0.05	+	+	+
	WP38	0.05	−	−	−
	WP52	0.05	−	−	+
Glucose	DSM 17395	0.22	+	+	+
	WP38	0.14	(+)	(+)	-
	WP52	0.13	(+)	(+)	+
Xylose	DSM 17395	0.13	+	+	+
	WP38	0.16	(+)	(+)	-
	WP52	0.15	(+)	(+)	+

OD₆₀₀, optical density at 600 nm.

Antimicrobial activity and pigmentation are scored relative to the wild-type strain as follows: +, similar; (+), reduced; −, none.

Homoserine lactone (HSL) production was evidenced as described by Berger et al. (3).