Fig 3.
Rapid and sensitive phage-mediated detection of P. cannabina pv. alisalensis BS91. (A) Signal response time. BS91 was grown at 28°C in NBY media, mixed with PBSPCA1::luxAB (time zero; 3.2 × 108 PFU/ml), and incubated at 28°C. Bioluminescence (RLU) was measured over time. Numbers represent means (n = 3) ± SD. (B) Sensitivity limit of detection. BS91 was grown to an OD600 of 0.15 (1.3 × 108 CFU/ml), 10-fold serially diluted, mixed with the reporter phage (3.2 × 108 PFU/ml), and incubated at 28°C for 120 min. Numbers represent means (n = 3) ± SD. *, significant increase (P < 0.05 [Student's t test]) compared to control results.