Fig 2.
Strain LO28 does not use the GADe system in BHI. (A) Concentration of GABAe measured at 80 min after acidification of the cultures to various pH values. The detection limit for GABAe quantification in BHI was 0.8 mM. (B) Acid resistance of stationary-phase cultures of wild-type (Wt) strains LO28 and 10403S following acid challenge at pH 2.7. All cultures were grown to stationary phase overnight at 37°C, and viability of cells was determined prior to acid challenge and every 20 min thereafter. Markers represent an average of measurements performed in triplicate, and error bars represent the standard deviation.