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. 2012 Jun;78(11):3966–3974. doi: 10.1128/AEM.07825-11

Fig 2.

Fig 2

Specificity and sensitivity analysis of PCR assay for C. sakazakii O1 to O7. (a) Agarose gel electrophoresis of the PCR products obtained from respective C. sakazakii O1 to O7 strains by using the multiplex PCR assay. Lanes 1 and 10, DL2000 DNA markers; lane 2, O1; lane 3, O2; lane 4, O3; lane 5, O4; lane 6, O5; lane 7, O6; lane 8, O7; lane 9, negative control. (b) Agarose gel electrophoresis of the PCR products obtained from serially diluted chromosomal DNA of C. sakazakii O6 strain G2704. Lanes 1 and 10, DL2000 DNA markers; lane 2, negative control; lane 3, 0.00001 ng; lane 4, 0.0001 ng; lane 5, 0.001 ng; lane 6, 0.01 ng; lane 7, 0.1 ng; lane 8, 1 ng; lane 9, 10 ng. (c) Agarose gel electrophoresis of the PCR products obtained from serially diluted culture of C. sakazakii O6 strain G2704. Lanes 1 and 9, DL2000 DNA markers; lane 2, negative control; lane 3, 101 CFU/ml; lane 4, 102 CFU/ml; lane 5, 103 CFU/ml; lane 6, 104 CFU/ml; lane 7, 105 CFU/ml; lane 8, 106 CFU/ml.