Table 6.
Characteristics of ORFs in the C. sakazakii O7 O-antigen gene clustera
| ORF | Gene | Gene position (length [aa]) | G+C content (%) | Pfam conserved domain(s) (Pfam no./e-value) | Similar protein(s), strain(s) (GenBank accession no.) | % Identical (aa overlap) | Putative function of protein |
|---|---|---|---|---|---|---|---|
| 7-1 | wzx | 69–1358 (430) | 33.02 | Polysaccharide biosynthesis protein (PF01943/8.6e-23) | Polysaccharide biosynthesis protein Wzx, V. vulnificus (ABD38622) | 31 (404) | Flippase |
| 7-2 | wepQ | 1286–2741 (485) | 29.42 | Glycosyltransferase group 1 (PF00534/5.1e-12) | Glycosyltransferase group 1, A. baumannii ABNIH2 (EGT89242.1) | 35 (264) | Glycosyltransferase |
| 7-3 | wepR | 2743–3918 (391) | 31.71 | Glycosyltransferase group 1 (PF00534/4.3e-13) | Glycosyltransferase group 1, Marinomonas sp. (YP_001339674.1) | 28 (392) | Glycosyltransferase |
| 7-4 | fnlA | 3911–4945 (344) | 41.06 | Polysacc_synt_2 (PF02719.8/2.7e-109) | l-Fucosamine synthetase, E. coli (ABM53659) | 93 (344) | Fucosamine synthetase |
| 7-5 | fnlB | 4947–6050 (367) | 41.30 | NAD-dependent epimerase/dehydratase family (PF01370.14/1.2e-13) | FnlB, epimerase, S. dysenteriae (ACA24843) | 78 (367) | Epimerase |
| 7-6 | fnlC | 6050–7180 (376) | 40.93 | UDP-N-acetylglucosamine 2-epimerase (PF02350.12/5.5e-95) | C-2 epimerase, E. coli MS 145-7 (ZP_07692194.1) | 92 (376) | Epimerase |
| 7-7 | wbuB | 7180–8388 (402) | 42.10 | Glycos_transf_1 (PF00534.13/1.6e-09) | l-Fucosamine transferase, S. dysenteriae (ACA24845) | 76 (398) | Glycosyltransferase |
| 7-8 | wbuC | 8379–8783 (135) | 42.71 | Domain of unknown function (DUF1971/0.0009) | WbuC, S. dysenteriae (ACA24846) | 69 (131) | Glycosyltransferase |
a
aa, amino acid(s).