Fig 1.
Different steps in vector construction, exemplified by the pRLYC8 vector. Features that are introduced at each step are shown in green. Unique restriction sites in the multiple cloning site (MCS) are shown in red. Other restriction sites of interest are shown in blue. bla is the beta-lactamase gene. ColE1 is the origin of replication for E. coli. pyrF and pyrE are genes taken from B. subtilis and fused to the PspacC promoter (which is shown as a boxed P). CAT194 is the S. aureus chloramphenicol resistance gene. repCts is the pT181ts replication gene. The full MCS is shown only in pFL and pRLYC8, but the region between the EcoRI and HindIII sites remains unchanged in all vectors.