Fig 4.

Comparison of two stereoisomers of cPY in inhibition of PLA₂ activity. PLA₂ was extracted from hemocytes of fifth-instar Spodoptera exigua as described in Materials and Methods. The hemocyte PLA₂ sample (10 μg) was incubated with 10 μl of bacterial culture broth at 25°C for 10 min. Then, the substrate solution was added and the residual PLA₂ activity was monitored at 348 nm for excitation and 390 nm for emission using a spectrofluorometer. Each measurement was replicated with three independent samplings. Error bars represent standard deviations.