Table 1.
Activation of various MazE-MazF fusion proteins by factor Xa, HIV-1 PR, and HCV NS3 protease
| Name | MazE regiona (aa) | Protease cleavage site | Expressionb | Toxicityc |
|---|---|---|---|---|
| MazEF-FXa | 42 to 82 | Factor Xa | +++ | − |
| MazE(61)F-FXa | 61 to 82 | Factor Xa | + | + |
| MazE(65)F-FXa | 65 to 82 | Factor Xa | − | +++ |
| MazE(71)F-FXa | 71 to 82 | Factor Xa | − | +++ |
| MazEF-HIV | 42 to 82 | HIV-1 PR | +++ | − |
| MazEF-HCV | 42 to 82 | HCV NS3 protease | +++ | − |
a
The residue numbers from the N-terminal end of MazE are shown (11).
b
A plus sign indicates expression, and three plus signs indicate very high expression of the fusion proteins. A minus indicates no expression.
c
A minus indicates that the fusion protein is not toxic even in the presence of IPTG. A plus sign indicates that it is toxic only in the presence of IPTG. Three plus signs indicate that it is toxic even in the absence of IPTG.