Table 1.
Genes differentially expressed after acid treatment of Listeria monocytogenes grown under various conditionsa
| Growth temp(s) and culture phase(s) prior to acid treatment | Upregulated genes |
Downregulated genes |
||
|---|---|---|---|---|
| No. | Examples | No. | Examples | |
| 7°C log phase only | 13 | lmo0066, lmo0320, lmo1151 | 6 | lmo0299, lmo0735, lmo1114 |
| 7°C stationary phase only | 28 | lmaA, lmaB, lmaD | 6 | lmo0049, lmo0728, lmo2445 |
| 37°C log phase only | 63 | clpC, grpE, clpP | 68 | lmo0680, lmo1406, minD |
| 37°C stationary phase only | 13 | rplU, lmo2362, rplP | 38 | lmo0770, murC, ndk |
| 7°C log phase + 7°C stationary phase | 3 | lmo2290, lmo2291, lmo2292 | 1 | lmo2210 |
| 37°C log phase + 37°C stationary phase | 0 | 5 | lmo0998, lmo1597, lmmo1965 | |
| 7°C log phase + 37°C log phase | 0 | 7 | lmo952, lmo1749, lmo2408 | |
| 7°C stationary phase + 37°C stationary phase | 1 | lmo2293 | 2 | lmo1049, atpI |
| 7°C log phase + 37°C stationary phase | 0 | 0 | ||
| 7°C stationary phase + 37°C log phase | 1 | lmo0903 | 1 | lmo1926 |
| 7°C log phase + 7°C stationary phase + 37°C log phase | 0 | 1 | lmo0883 | |
| 7°C log phase + 7°C stationary phase + 37°C stationary phase | 0 | 0 | ||
| 37°C log phase + 37°C stationary phase + 7°C log phase | 0 | 1 | lmo1639 | |
| 37°C log phase + 37°C stationary phase + 7°C stationary phase | 0 | 3 | lmo0217, lmo1828, deoD | |
| All conditions tested | 0 | 0 | ||
| Total | 122 | 139 | ||
a
Genes showing a change in expression with an absolute value of ≥1.5-fold and an adjusted P value of ≤0.05 (LIMMA) after acid treatment for 5 and/or 15 min (acid treatment was BHI-MOPS adjusted to pH 3.5 with HCl followed by incubation at 37°C).