Table 1.
Strain, plasmid, or primer | Origin, characteristic, or sequence | Sourcea |
---|---|---|
C. sakazakiistrains (no.) | ||
NCTC08155 (1) | DPC | |
ATCC 12868 (2) | ATCC | DPC |
ATCC 29004 (3) | ATCC | DPC |
DSM4485 (4) | DPC | |
NCTC11467 (5) | DPC | |
DPC 6522 (6) | Blood | DPC |
DPC 6523 (7) | Cerebrospinal fluid | DPC |
DPC 6524 (8) | Stool | DPC |
DPC 6525 (9) | Urine | DPC |
DPC 6526 (10) | Blood | DPC |
DPC 6527 (11) | Blood | DPC |
DPC 6528 (12) | Cerebrospinal fluid | DPC |
DPC 6529 (13) | Tracheal aspirate | DPC |
DPC 6530 (14) | Bronchial alveolar lavage fluid | DPC |
ATCC BAA-894 (15) | ATCC | DPC |
ATCC BAA-894 + rpoSb | This study | |
DPC 6523 + rpoSb | This study | |
Plasmid | ||
pNZ44 | Cmrc | 43 |
Primers | ||
RPOS-1 | TGATTACCTGAGTGCCTACG | |
RPOS-2 | TGAACTTCATGAGGGAGAGC | |
RPOS-3 | GATCGAAAGTAACCTGCGTC | |
RPOS-4 | TCCGGAACCAGTTCAACACG | |
RPOS-5 | CGCAGATAGACGTTAAGCTC | |
RPOS-6 | TGTCTGGCGGCATGTAATTC | |
RPOS-7 | CTAGCCATGGATCAGAATACGCTGAAAG | |
RPOS-8 | GTCATCTAGAGTGATTATTCGCGGAACAG |
All strains were obtained from the Dairy Products Research Centre (DPC), Fermoy, County Cork, Ireland. Strains 6 to 14 were originally obtained from M. Ardino, Centers for Disease Control and Prevention, Atlanta, GA.
rpoS was amplified from C. sakazakii DPC 6529.
Cmr, chloramphenicol resistance.