Table 1.
Bacterial strains, plasmids, and primers
| Strain, plasmid, or primer | Origin, characteristic, or sequence | Sourcea |
|---|---|---|
| C. sakazakiistrains (no.) | ||
| NCTC08155 (1) | DPC | |
| ATCC 12868 (2) | ATCC | DPC |
| ATCC 29004 (3) | ATCC | DPC |
| DSM4485 (4) | DPC | |
| NCTC11467 (5) | DPC | |
| DPC 6522 (6) | Blood | DPC |
| DPC 6523 (7) | Cerebrospinal fluid | DPC |
| DPC 6524 (8) | Stool | DPC |
| DPC 6525 (9) | Urine | DPC |
| DPC 6526 (10) | Blood | DPC |
| DPC 6527 (11) | Blood | DPC |
| DPC 6528 (12) | Cerebrospinal fluid | DPC |
| DPC 6529 (13) | Tracheal aspirate | DPC |
| DPC 6530 (14) | Bronchial alveolar lavage fluid | DPC |
| ATCC BAA-894 (15) | ATCC | DPC |
| ATCC BAA-894 + rpoSb | This study | |
| DPC 6523 + rpoSb | This study | |
| Plasmid | ||
| pNZ44 | Cmrc | 43 |
| Primers | ||
| RPOS-1 | TGATTACCTGAGTGCCTACG | |
| RPOS-2 | TGAACTTCATGAGGGAGAGC | |
| RPOS-3 | GATCGAAAGTAACCTGCGTC | |
| RPOS-4 | TCCGGAACCAGTTCAACACG | |
| RPOS-5 | CGCAGATAGACGTTAAGCTC | |
| RPOS-6 | TGTCTGGCGGCATGTAATTC | |
| RPOS-7 | CTAGCCATGGATCAGAATACGCTGAAAG | |
| RPOS-8 | GTCATCTAGAGTGATTATTCGCGGAACAG |
a
All strains were obtained from the Dairy Products Research Centre (DPC), Fermoy, County Cork, Ireland. Strains 6 to 14 were originally obtained from M. Ardino, Centers for Disease Control and Prevention, Atlanta, GA.
b
rpoS was amplified from C. sakazakii DPC 6529.
c
Cmr, chloramphenicol resistance.