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(A) Micrographs of HA-Gfp-tagged pheromone receptor localization in dikaryotic S. commune transformants by immunostaining (Aa to Ad) and in vivo (Ae and Af) in dikaryotic mycelium (Vbar2tG1 or Vbar2tG11 mated to 4-39); (B) differentiation of pseudoclamps showing strong receptor staining while others are nonfluorescent (top, bright field; middle, DAPI and calcofluor; bottom, Gfp). Bars, 5 μm.
