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Localization of TY-YFP-RPA2 in bloodstream-form cells. Cells containing one tagged allele and a second which is either wild type (A) or a knockout (B) are shown. Localization is unaffected by the absence of untagged protein, showing incorporation into both the nucleolus and the ESB (arrows). YFP fluorescence is pseudocolored in green, DAPI in blue. Bars, 1 μm.
