Fig 5.

Effects of ODAL1 silencing on ciliary orientation in response to Ca²⁺ and cAMP. The ciliary orientation in response to Ca²⁺ and cAMP was determined in the presence of 30% glycerol. The demembranated cortical sheets from control, ODAL1-RNAi (2 days after the induction of gene silencing), and ND7-RNAi (2 days after the induction of gene silencing) cells were perfused successively with reactivation solutions containing 30% glycerol, 1 mM ATP, 1 mM MgCl₂, 50 mM potassium acetate, and 10 mM Tris-maleate buffer (pH 7.0) and various concentrations of free Ca²⁺ and cAMP, as noted in the abscissas. The free Ca²⁺ concentration in the reactivation solutions was controlled as indicated in Materials and Methods. The orientation between 11 o'clock and 2 o'clock indicates a Ca²⁺-induced ciliary reversal. (A) Changes in ciliary orientation in response to Ca²⁺ without cAMP. (B) Changes in ciliary orientation in response to cAMP in the presence of 2 μM Ca²⁺. Values represent means ± SD (n = 5 to 6).