Fig 5.

Phenotype of the MTLa/MTLa Δ/Δ myo5 MET3-WOR1 mutant strain. (A) MTLa/MTLa MET3-WOR1 Δ/Δ myo5 cells growing on solid medium under MET3 inductive conditions (SCD medium lacking Met and Cys) can exhibit a morphology characteristic of opaque cells (black arrows) or can form aberrant hypha-like structures (blue arrows). (B) Cells are unable to sustain the opaque cell morphology under MET3 shutoff conditions (overnight growth in SCD liquid medium) and switch back to the white phenotype. Nomarski images are shown. Bars, 5 μm.