Fig 6.

Mating assay for the MTLa/MTLa Δ/Δ myo5 MET3-WOR1 strain. Cells were placed on plates containing SCD-MC or SCD+MC medium. After 4 to 5 days of growth at 24°C, cells from randomly chosen colonies were mated with tester strain 3315 (MTLα trp1/trp1 lys2/lys2) on SCD-MC or SCD+MC plates, respectively. The wild-type strain 3294 (MTLa Δura3::imm34/Δura3::imm434 his1/his1 arg5,6/arg5) was used as a control. Afterwards, plates were printed on selective SCD-MC medium (A) or on SCD+MC medium lacking histidine and lysine (B). Activation of WOR1 expression allowed all cells to mate.