Fig 7.

Phenotype of MTLa/MTLa MET3-MYO5/Δmyo5 mutant cells. (I) (A) On SCD+MC medium, which suppresses the expression of MYO5, cells exhibit an abnormally round shape, with some cells enlarged, and the cells are clumped, characteristic of white-form myo5 mutants. (B) On SCD-MC medium, which allows MYO5 expression, the cells have a normal size and shape and are dispersed. An upright microscope was used. Magnification, ×90. Nomarski images are shown. Bar, 10 μm. (II) (A) MTLa/MTLa MET3-MYO5/Δmyo5 cells grown on SCD-MC medium can switch from the white to the opaque morphology. (B and C) MTLa/MTLa MET3-MYO5/Δmyo5 opaque cells grown on SCD+MC solid medium with phloxine B. (B) Opaque and hypha-like cells (from “wrinkled” colonies). (C) Clumped cells of abnormal shape (from “smooth” colonies). An inverted microscope was used. Magnification, ×40. Bar, 12.7 μm.