Table 3.
PCR primers used in this study
| Primer | Sequence (5′–3′)a | Source |
|---|---|---|
| MYO5-Ca MET3F1 | TTTCTTCTTCTTTTATGACAAACATTTAAGATTTAATTCACATTTCCATTCATCAACTAATTAAACCTCTAGTCATTATCATTATTTATTTCATTtaatcgctctggcgcaggtgctt | This study |
| MYO5-CaMET3R1 | CGGCTTTTTTGATGCCACCACTACTACCACACCCAGAAGATTTGGCAGGTACTTGTTGTTGTTTTGTTTTGGTACGACCACCTCTTTTCACAATAGCCATtctgatatcatcgatgaattcgag | This study |
| SLA2 F1 | ACCATTACTCCCCCTAATATATTGTTAAACACTTTGCCCCCCCCCCCCCCCCTTAGCCCAtgtggaattgtgagcggata | This study |
| SLA2 F2 | AGAGAGACAGAAGAAATGAACACAACGAAAAAAAGAGCTCCGCCTATTAACTTATAACATACCATTACTCCCCCTAATA | This study |
| SLA2 R1 | GTGAATTATGGGTGGAAACAGAATATTATGATGAATGGAAACAAAGAATTCAAATACTTGgttttcccagtcacgacgtt | This study |
| SLA2 R2 | TCCCAAAATTGGCAAATTTATCATTAAAACAAGTACTTAATCCTAAAATTAGTGAATCTTGTGAATTATGGGGTGGAAACA | This study |
| ACT1 Fb | GAAGCCCAATCCAAAAGAGGT | 4 |
| ACT1 Rb | CTTCTGGAGCAACTCTCAATTC | 4 |
| WOR1 Fb | TGAACCCATGCCAATGACTA | This study |
| WOR1 Rb | GCTGCTGCAGTTGATGTTGT | This study |
| MYO5Fb | AACCGGCATATCCAATACCA | This study |
| MYO5Rb | TTGTAAGCTTGGTGCTGGTG | This study |
a
Capital letters indicate homology to genomic DNA. Lowercase letters indicate homology to plasmids pFA and pGEM.
b
Quantitative PCR primer.