Fig 2.
Representative anaerobic batch fermentation of C. glutamicum ΔldhA/pAN6-pycP458S (♦; biomass concentration, 6.35 g cdw/liter), C. glutamicum BOL-1/pAN6-pycP458S (▲; biomass concentration, 6.00 g cdw/liter), and C. glutamicum BOL-2 (■; biomass concentration, 5.33 g cdw/liter) showing glucose consumption (A), succinate formation (B), acetate formation (C), and pyruvate formation (D). The experiments were performed in a Multifors bioreactor system, and the cells, pregrown aerobically, were resuspended in a solution containing 0.9% (wt/vol) NaCl solution, 111 mM glucose, and 200 mM NaHCO3. The pH was kept constant at 6.9 by addition of 3 M KOH. Note that the different rates cannot be directly compared, as the biomass concentrations differed. The average values of the final process parameters, including standard deviations from three independent fermentations for each strain, are displayed in Table 2.