Table 1.
Characteristics of the seven S. kudriavzevii microsatellite loci
| Locus | Closest ORF in S. cerevisiae | No. of repeats in IFO1802 | No. of alleles detected | Size range (bases) | Primer |
|
|---|---|---|---|---|---|---|
| Orientationa | Sequence (5′–3′) | |||||
| SKC4 | YOL109W (ZEO1) | (TAA)17 | 4 | 230–242 | F | AAGAATGGTGGAAAGATGCTGT |
| R | TCTTTTTCTCTCCTGCGTCAGA | |||||
| SKTAA1 | YGL035c (MIG1) | (TAA)20 + (TAA)10 | 12 | 284–374 | F | AGAAGCGGAAACACTACCGCCTAT |
| R | TGTATGTTTGTCAAAAGCATCCAC | |||||
| SKYPL009c | YPL009c | (CTT)16 | 8 | 162–258 | F | TGCGACACCATCCGCATTTTC |
| R | ATGGAAAGTGAAGACAGGTGA | |||||
| SKCTT2 | YJL123c (MTC1) | (CTT)11 | 4 | 267–285 | F | TAGCATGGCGAATGGAGATC |
| R | ACGAGGCCGACGGCAGCTCCT | |||||
| SKCTT3 | YKL201c (MNN4) | Uneven 7(CTT)3 | 12 | 299–445 | F | CATCTTCTTGTCTGTTTTTGC |
| R | AAGATTATGCGTATGGGAAA | |||||
| SKGTT1 | YJL206c | (GTT)17 | 5 | 116–173 | F | TGGACCGGAGACTTGTGGCT |
| R | TTTGACGGTCCAACAGCAA | |||||
| SKGTT2 | YCR093w (CDC39) | (GTT)16 | 9 | 188–286 | F | CCGAACAAATAAGAGCTCAA |
| R | GCTGATTGAGGAGGTTGAT | |||||
a
F, forward; R, reverse.