Table 2.
Detection of S. kudriavzevii DNA in the genomes of three S. cerevisiae/S. kudriavzevii hybrids
| Chromosome | Locusa (accession no.) | Enzyme | Profileb |
||
|---|---|---|---|---|---|
| Eg8/136 | El1d4 | Uvaferm CEG | |||
| 1 | YAL008W | DraI or HindIII | Sc+Sk | Sc+Sk | Sc |
| 2 | YBL021C | ClaI or PstI | Sc+Sk | Sc+Sk | Sc+Sk |
| 3 | SK_YCR093w | Sk | Sk | ND | |
| YCL014w | EcoRI | Sc | Sc+Sk | Sc | |
| 4 | YDL003W | ClaI or SmaI | Sc+Sk | Sc+Sk | Sc |
| YDR337fw | DraI | Sc+Sk | Sc+Sk | Sc | |
| 5 | YEL061wfw | HaeIII | Sc+Sk | Sk+Sc | Sc |
| YER073w (HE585129) | Sc+Sk | ||||
| 6 | YFR030W | HindIII or BamHI | Sc+Sk | Sc+Sk | Sc+Sk |
| 7 | SK_YGL035 | Sk | Sk | ND | |
| YGL162W | HindIII | Sc+Sk | Sc+Sk | Sc | |
| 8 | YHL038c | HaeIII | Sc+Sk | Sc+Sk | Sc |
| YHR043C | SacI or HindIII | Sc | Sc+Sk | Sc | |
| 9 | YIL013C | SacI or BamHI | Sc+Sk | Sc+Sk | Sc+Sk |
| 10 | SK_YJL206c | Sk | Sk | ND | |
| SK_YJL123c | Sk | Sk | ND | ||
| YJL052W | ClaI | Sc+Sk | Sc+Sk | Sc | |
| YJR139c | DraI | Sc+Sk | Sc+Sk | ND | |
| 11 | YKL004W | HindIII | Sc+Sk | Sc | Sc |
| 12 | YLR001C | EcoRI | Sc+Sk | Sc+Sk | Sc |
| 13 | YMR001c | BamHI | Sc+Sk | Sc+Sk | Sc+Sk |
| YMR303w | HaeIII | Sc+Sk | Sc+Sk | Sc | |
| 14 | YNL291c | HindIII or HinfI | Sc+Sk | Sc+Sk | Sc |
| YNR001C | HindIII | Sc+Sk | Ndet | Sc | |
| 15 | SK_YOL109w | Sk | Sk | ND | |
| YOL002C | SacI | Sc+Sk | Sc+Sk | Sc | |
| YOR374w (HE585131) | Sc+Sk | ||||
| 16 | SK_YPL009w | Sk | Sk | ND | |
| YPL001W | EcoRV | Sc+Sk | Sc+Sk | Sc | |
| YPL061w (HE585130) | Sc+Sk | ||||
| YPR140 | HinfI | Sc+Sk | Sc+Sk | Sc | |
a
S. kudriavzevii microsatellite loci are indicated by an “SK_” prefix. For the locus YCL014w, ARD6.1 (a French isolate) was used in place of IFO1802 as a reference.
b
Sk, Saccharomyces kudriavzevii; Sc, Saccharomyces cerevisiae; ND, not detected; Ndet, not determined.