Table 2.

Proteins identified as differently expressed in CZR relative to NP1

Spot IDa	Protein descriptionb	Gene locusc	Gene name	NCBI accession no.	Theoretical Mr	Theoretical pI	MASCOT score	Sequence coverage (%)	No. of peptides detected by MS-MS
Overexpressed proteins
1	Argininosuccinate synthetase	SSCG_05695	argH	gi:254393199	43,536	4.88	420	32	5
2	N-Acetyl-gamma-glutamyl-phosphate reductase	SSCG_05690	argC	gi:254393193	35,652	5.6	309	54	5
3	Carboxyethylarginine synthase	SSCG_00150	ceaS2	gi:254387584	61,040	5.04	398	45	5
4	Proclavaminate amidino hydrolase	SSCG_00152	pah2	gi:254387586	33,494	5.69	614	65	6
5	Carboxyethyl-arginine beta-lactam-synthase	SSCG_00151	bls2	gi:254387585	54,616	5.92	264	42	4
6	Glutamate N-acetyltransferase 2	SSCG_00154	oat2	gi:254387588	41,810	4.73	428	27	5
7	Acyl-CoA dehydrogenase domain-containing protein	SSCG_03485	holB	gi:254390828	42,720	6.16	209	46	3
8	Glucose-methanol-choline oxidoreductase	SSCG_03487	holD	gi:254390830	59,338	5.8	573	72	6
9	Lantibiotic biosynthesis protein	SSCG_03489	holF	gi:254390832	21,323	5.8	475	82	5
10	Globin	SSCG_03490	holG	gi:254390833	17,779	5.46	181	49	5
11	Thioredoxin-disulfide reductase	SSCG_03492	holI	gi:254390835	32,572	5.6	330	70	5
12	Cysteine synthase	SSCG_02363	cysM	gi:254389812	33,844	5.64	536	68	6
13	Thiosulfate sulfurtransferase	SSCG_01126	rhlA	gi:254388565	31,710	4.68	661	50	6
14	Thioredoxin	SSCG_04064		gi:254391438	11,899	5.01	346	78	4
15	Thiamine S protein	SSCG_02362		gi:254389811	10,096	4.47	454	63	6
16	S-Adenosyl-l-homocysteine hydrolase	SSCG_04409		gi:254392040	51,809	4.94	533	38	5
17	DsbA oxidoreductase	SSCG_05980		gi:254393490	23,671	4.7	340	62	5
18	Acetyl-CoA synthetase	SSCG_04276	acs	gi:254391696	71,277	5.16	480	50	6
19	Acyl-CoA dehydrogenase	SSCG_07331		gi:254392359	42,153	5.52	616	73	6
20	Cephalosporin hydroxylase	SSCG_00138	cmcI	gi:254387571	27,738	4.8	469	81	4
21	Deacetoxycephalosporin C synthetase	SSCG_00134	cefE	gi:254387568	34,933	5.14	239	46	5
22	Pyrimidine regulatory protein	SSCG_03803	pyrR	gi:254391342	20,734	6.32	199	74	3
23	UDP-glucose 4-epimerase	SSCG_00066	tunF	gi:254387500	35,355	5.58	222	56	3
24	Bromoperoxidase-catalase	SSCG_01385		gi:254388967	54,190	5.53	592	48	6
Underexpressed proteins
25	Carboxyethyl arginine synthase isoenzyme 1	SSCG_00629	ceaS1	gi:254388064	59,381	4.8	423	50	5
26	Conserved hypothetical protein	SSCG_06975	cvm2	gi:294813756	16,923	5.26	305	71	4
27	Malate dehydrogenase	SSCG_05972		gi:254393481	34,622	5.1	639	68	4
28	Serine hydroxymethyltransferase	SSCG_00627	orfA	gi:254388063	42,014	5.81	217	38	4
29	BldKD	SSCG_02719		gi:254390253	38,100	5.56	242	34	4
30	YjgF family regulator	SSCG_00626	orfB	gi:254388062	13,498	4.63	408	75	4
31	Beta-lactamase domain-containing protein	SSCG_00563		gi:254387999	70,690	4.9	644	56	3
32	Guanylate kinase	SSCG_03810		gi:254391349	11,498	10.4	306	83	3
33	Bacterial luciferase domain-containing protein	SSCG_08125		gi:294817230	36,947	10.6	532	51	5
34	O-Acetylhomoserine aminocarboxypropyltransferase	SSCG_06530		gi:294816384	27,654	6.92	111	27	2
35	Protease	SSCG_05363		gi:254392867	20,497	5.11	230	58	5
36	Beta-ketoadipyl CoA thiolase	SSCG_06569		gi:294816329	40,945	5.9	456	76	6
37	Methylenetetrahydromethanopterin reductase	SSCG_00780		gi:254388217	38,232	5.34	212	27	5

^aSpot ID numbers correspond to the numbers in the 2D images in Fig. 6.

^bThe functional descriptions of identified proteins are based on the genome annotation of S. clavuligerus ATCC 27064 of the Broad Institute.

^cGene loci were obtained from the genome of S. clavuligerus ATCC 27064 at http://www.broadinstitute.org/ and NCBI.