Fig 3.

Glyoxylate carboligase activity in strain CB1190 and strain RHA1/pTip cell extracts. (A) Phosphate buffer or cell extracts from pyruvate- or dioxane-grown strain CB1190 cells were amended with 10 mM glyoxylate, and then the amount of glyoxylate remaining and CO₂ production were determined. (B) The same assay as described for panel A was performed with cell extracts prepared from strain RHA1 cells with pTip-Psed_3890 (3890; putative glyoxylate carboligase), pTip-3889 (3889; putative tartronate semialdehyde reductase), a mix of the two (3890 + 3889), pTip-QC2 (empty vector), or buffer.