Table 2.
Normalized relative expression levels for selected strain CB1190 genes analyzed by qRT-PCR
| Gene group | Gene ID | Gene name | Gene product | Replicona | Expression ratiob |
|
|---|---|---|---|---|---|---|
| Dioxane/pyruvate | Glycolate/pyruvate | |||||
| Monooxygenase genes lacking probes on microarray | Psed_6976 | thmA | THF monooxygenase α-subunit | p2 | 15 ± 0.2 | 1.0 ± 0.5 |
| Psed_0629 | prmA | Methane monooxygenase | c | 0.4 ± 0.1 | 0.8 ± 0.5 | |
| Psed_0768 | Phenol 2-monooxygenase | c | 2 ± 2 | 0.9 ± 0.4 | ||
| Psed_0815 | Methane/phenol/toluene hydroxylase | c | 1.1 ± 0.5 | 0.3 + 0.4 | ||
| Dioxane metabolism intermediate-related genes | Psed_6782 | Alcohol dehydrogenase | p1 | 7.3 ± 0.8 | 3.3 ± 0.4 | |
| Psed_6783 | Fatty-acid-CoA ligase | p1 | 24 ± 2 | 7 ± 5 | ||
| Psed_6970 | FAD/FMN-dependent dehydrogenase | p2 | 6.1 ± 0.8 | 1.5 ± 0.9 | ||
| Psed_6971 | Alcohol dehydrogenase | p2 | 8.7 ± 0.8 | 1.3 ± 0.4 | ||
| Psed_6975 | Aldehyde dehydrogenase | p2 | 23 ± 2 | 0.9 ± 0.3 | ||
| Psed_6981 | Aldehyde dehydrogenase | p2 | 11 ± 1 | 1.5 ± 0.9 | ||
| Glycolate and glyoxylate metabolism-related genes | Psed_4783 | glcD1 | Glycolate oxidase | c | 0.3 ± 0.1 | 0.7 ± 0.5 |
| Psed_4788 | glcD2 | Glycolate oxidase | c | 3.6 ± 0.3 | 2.8 ± 0.5 | |
| Psed_4782 | glcB | Malate synthase G | c | 5.4 ± 0.2 | 2 ± 1 | |
| Psed_3888 | hyi | Hydroxypyruvate isomerase | c | 5 ± 1 | 3.1 ± 0.3 | |
| Psed_3889 | glxR | Tartronate semialdehyde reductase | c | 13 ± 1 | 6 ± 2 | |
| Psed_3890 | gcl | Glyoxylate carboligase | c | 18 ± 2 | 10 ± 4 | |
| Psed_3891 | glxK | Glycerate kinase | c | 1.9 ± 0.6 | 1.9 ± 0.7 | |
a
c, chromosome; p1, plasmid pPSED01; p2, plasmid pPSED02.
b
Genes upregulated with dioxane but not with glycolate are indicated in boldface.