Fig 6.

PMP content, OD, cell dry weight, and supernatant analysis during the time course of the cultivation experiment. A. mimigardefordensis strains SHX5 and SHX13 were first incubated in modified MS medium containing 2 g/liter NH₄Cl and 7 g/liter glycerol at the first cultivation stage. After their OD had reached 500 Klett units, all cells were transferred into the same MS medium containing 7 g/liter glycerol plus 9 g/liter DTDP as a precursor. (A) Increase of OD (Klett unit) and cell dry weight in the second cultivation stage. Symbols: ● and ○, turbidity increase of SHX5 and SHX13; ▲ and △, cell dry weight increase of SHX5 and SHX13. (B) PMP content over time in the second-stage cultivation stage. Symbols: ★ and ☆, strains SHX5 and SHX13. (C) Concentrations of DTDP, 3MP, and glycerol in different culture supernatants in the second-stage cultivation stage. Symbols: ▼ and ▽, consumption of DTDP in cultures of SHX5 and SHX13; ■ and □, degradation of glycerol in cultures of SHX5 and SHX13; ♦ and ♢, occurrence of 3MP in cultures of strains SHX5 and SHX13.