Table 2.
Relationships between PMP production and phaCs from different sourcesa
| Strain | PMP content (%) (wt/wt [CDW]) | CDW without PMP wt (g/liter) | Concn (g/liter) in supernatant of: |
||
|---|---|---|---|---|---|
| Glycerol | 3MP | DTDP | |||
| SHX6 | 7.02 ± 0.06 | 4.34 ± 0.41 | 0.6 ± 0.9 | — | 3.8 ± 0.3 |
| SHX15 | 0 | 3.10 ± 0.17 | 6 ± 0.8 | 0.48 ± 0.00 | 3.7 ± 0.9 |
| SHX18 | 13.38 ± 0.14 | 4.21 ± 0.08 | — | — | 3.6 ± 0.1 |
| SHX19 | 12.76 ± 1.26 | 4.35 ± 0.32 | — | — | 3.9 ± 0.8 |
| SHX20 | 4.49 ± 0.40 | 3.30 ± 0.35 | 4.9 ± 0.5 | 0.4 ± 0.01 | 4.4 ± 0.2 |
| SHX21 | 4.31 ± 0.23 | 3.53 ± 0.15 | 4.9 ± 0.5 | 0.34 ± 0.06 | 4.7 ± 0.3 |
a
The strains were first incubated in modified MS medium containing 2 g/liter NH4Cl, 10 g/liter glycerol, and 0.4 g/liter yeast extract. A 1 mM final concentration of IPTG was added when the OD600 reached 0.6 (early exponential phase). After the cells arrived at late exponential phase (OD600 of approximately 4.3), they were collected and transferred into the same medium supplied with 7 g/liter DTDP as a precursor. After 4 days of incubation, all samples were analyzed for PMP content by GC analysis. CDW, OD600, and supernatant of the final cultures were also measured. Values are means and standard deviations from three independent experiments. —, below detection limit.