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Effect of pH and temperature on the activities of EGPh and mutants. Enzyme activities were tested with 1% CMC for 1 h. (A) pH optima were measured at 80°C for EGPh and N-terminal deletion mutants and at 70°C for mutants containing C-terminal deletions. (B) Temperature optima were determined with 100 mM acetate buffer (pH 5.5). Each point represents the mean ± the standard deviation of triplicate experiments. The data were normalized to the maximum enzymatic activity.
