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SDS-PAGE of the crude extract from E. coli Rosetta 2(DE3)(pET-22b-StrepII-CSN-eGFP-HIS6 E122Q, pLysSRARE2); (60 μg, lane 1) and CAP purified using affinity chromatography (6 μg; lane 2), either stained using Coomassie brilliant blue G-250 (A) or Western blotted and detected by chemiluminescence using HRP-coupled StrepII affinity protein (B). The band at ca. 75 kDa represents CAP. M, molecular mass markers.
