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Dot activity assay to check the enzymatic activity of wild-type chitosanase and CAP. Lane 1, crude extract from E. coli Rosetta 2(DE3)(pET-22b(+), pLysSRARE2); lane 2, purified wild-type chitosanase; lane 3, purified CAP. Chitosanase activity is revealed as a dark spot in the calcofluor white-stained gel.
