Fig 5.

Double staining of in vitro-induced infection structures of the wheat stem rust fungus Puccinia graminis f. sp. tritici. 1, spore; 2, germ tube; 3, appressorium; 4, substomatal vesicle; 5, infection hypha. Germ tube, appressorium, and the tip of the infection hypha were labeled by WGA conjugated to Texas Red (red fluorescence), indicating the presence of chitin, while CAP staining revealed the presence of chitosan in the substomatal vesicle and, less marked, infection hypha (green fluorescence).