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Scheme of the reaction catalyzed by nucleoside phosphorylases. (A) Phosphorolytic and the synthetic pathways. (B) Synthesis of nucleosides by coupled NP activity reactions. A donor nucleoside is cleaved by an NP, yielding a free nucleobase (B1) and a pentose-1-P. The pentose is then used in a new reaction by either the same or a different NP, along with an acceptor nucleobase (B2) to synthesize a new nucleoside.
