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Purification of proteins TTC0188, TTC1491, TTC0194, TTC1070, and TTC1412. The indicated proteins were overproduced from the corresponding pET22b derivatives in E. coli BL21 cells. Lanes: 1, whole extract obtained after cell disruption by sonication; 2, soluble fraction after incubation of the whole extract at 80°C for 1 h; 3, purification by ion-exchange chromatography of the heat-treated extract; M, size markers (in kilodaltons).
