Table 1.
Oligonucleotides used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| TTC0188F | TTCCATATGGTGTGGCTCCTCCTTTCCCCCA |
| TTC0188R | TTCGAATTCTCAGCCGGGGGGCCTACG |
| TTC0194F | TTCCATATGATGCCGGATATGGAGCTC |
| TTC0194R | TTCGAATTCTCAGAGCTCGGCGAGGAT |
| TTC1185F | TTCCATATGGTGACCGCCTTCTTCGCCGCCGA |
| TTC1185R | TTCGAATTCTCACTCTACCAGGGCCCGGGCGA |
| TTC1070F | TTCCATATGATGAGCCCCATCCACGTG |
| TTC1070R | TTCGAATTCTCAAACCTCCAGGACGGC |
| TTC1412F | TTCCATATGATGAACCCCGTGGTCTTCATCCGGGA |
| TTC1412R | TTCGAATTCCTAGATGGCCTCCAGGACCAAGGG |
| TTC1491F | TTCCATATGATGGACGCGGTGAAGAAGGCCA |
| TTC1491R | TTCGAATTCCTAGGCCCTGAGAAAGGCCACG |
a
Restriction sites for NdeI (CATATG) and EcoRI (GAATTC) are indicated in italics, and the nucleotide excess sequences for proper anchoring of the restriction enzymes are underlined.