Table 5.
Kinetic parameters of NPsa
| Substrate | TtPNPI |
TtPNPII |
TtPyNP |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Km (mM) | kcat (s−1) | kcat/Km | Km (mM) | kcat (s−1) | kcat/Km | Km (mM) | kcat (s−1) | kcat/Km | |
| Guo | 0.53 ± 0.01 | 296 ± 2 | 558.78 | ||||||
| dGuo | 1.01 ± 0.03 | 830 ± 6 | 822.53 | ||||||
| Ino | 2.08 ± 0.02 | 173 ± 2 | 83.20 | ||||||
| dIno | 2.06 ± 0.02 | 627 ± 6 | 304.33 | ||||||
| Ado | 0.32 ± 0.02 | 440 ± 3 | 1,376.47 | ||||||
| dAdo | 0.36 ± 0.03 | 563 ± 4 | 1,563.53 | ||||||
| Urd | 0.12 ± 0.01 | 202 ± 6 | 1,683.33 | ||||||
| dUrd | 0.36 ± 0.3 | 562 ± 17 | 1,561.11 | ||||||
| Thd | 0.42 ± 0.02 | 400 ± 12 | 952.38 | ||||||
a
Calculated kinetic parameters of T. thermophilus HB27 NPs with different substrates at 80°C. Values are presented as means ± the standard deviations where applicable. Acronyms: Guo, guanosine; dGuo, 2′-deoxyguanosine; Ino, inosine; dIno, 2′-deoxyinosine; Ado, adenosine; dAdo, 2′-deoxyadenosine; Urd, uridine; dUrd, 2′-deoxyuridine; Thd, thymidine.