Fig 3.

Molecular characterization of transformants of Parietichytrium sp. TA04Bb and Schizochytrium sp. SEK 579. (A) G418-resistant phenotypes of transformants generated by transformation with Ubi-Neo^r. Transformants were inoculated on PD agar plates containing G418 at the concentrations indicated, with wild-type strains used as a control. Incubation was carried out at 25°C for 7 days. (B) Genomic PCR detecting neo^r in transformants generated by transformation with Ubi-Neo^r. The PCR product corresponding to neo^r is 0.8 kb. Lanes: M1, bacteriophage λ HindIII digest (marker); M2, phage ϕX174 HincII digest (marker); T, transformants (each number corresponds to the transformant's number); W, wild-type strain; P, positive control (Ubi-Neo^r was used as the PCR template). Details are given in Materials and Methods.