Fig 6.

Gene disruption of Δ5 desaturase in T. aureum ATCC 34304. (A) Scheme for disruption of the Δ5 desaturase gene in T. aureum ATCC 34304 (TauΔ5des) by TauΔ5desKoNeo^r (targeting construct for 1st allele) and TauΔ5desKoHyg^r (targeting construct for 2nd allele). Annealing sites for PCR primers are shown. (B) Reverse transcription-PCR detecting the TauΔ5des mRNA in the wild type and TauΔ5des-disrupted mutant (ΔtauΔ5des) of T. aureum. (C) GC analysis of FAMEs from the wild type and TauΔ5des-disrupted mutant (ΔtauΔ5des) of T. aureum. Details are given in Materials and Methods.