Table 2.
Transformation frequencies of thraustochytridsa
| Strain | Transformation method | No. of colonies per 1 μg vector DNA |
|---|---|---|
| A. limacinum mh0186 | Microprojectile bombardment | Extremely rare |
| Electroporation | 1.6 × 102 | |
| T. aureum ATCC 34304 | Microprojectile bombardment | 1.9 × 102 |
| Electroporation | Extremely rare | |
| Parietichytrium sp. TA04Bb | Microprojectile bombardment | 5.0 × 101 |
| Electroporation | 0 | |
| Schizochytrium sp. 204-06 m | Microprojectile bombardment | 4.6 × 101 |
| Electroporation | 0 |
a
Ubi-Neor was transfected into cells by both electroporation and particle bombardment, followed by incubation on PD agar plates containing 0.5 mg/ml (A. limacinum mh0186) or 2 mg/ml (T. aureum ATCC 34304, Parietichytrium sp. TA94Bb, and Schizochytrium sp. 204-06 m) of G418 at 25°C until G418-resistant colonies were formed. The number of transformants was then determined by counting the colonies on the plates.