Table 2.
Summary of all the culture conditions used to examine pesL and pes1 expression
| Gene and culture conditiona | Time period (h) | NRPS expressedb |
|---|---|---|
| pesL | ||
| MEM supplemented with 5% FCS | 24 | Y |
| 48 | Y | |
| 72 | Y | |
| 96 | Y | |
| YG medium | 24 | N |
| 48 | Y | |
| 72 | Y | |
| 96 | N | |
| Czapek's medium | 24 | Y |
| 48 | Y | |
| 72 | Y | |
| 96 | Y | |
| AMM | 24 | Y |
| 48 | N | |
| pes1 | ||
| Sabouraud broth | 24 | Y |
| 48 | Y | |
| 72 | N | |
| MM | 24 | Y |
| 48 | Y | |
| 72 | N | |
| AMM | 24 | Y |
| 48 | Y | |
| 72 | Y |
a
MEM, minimal Eagle medium; FCS, fetal calf serum; YG, yeast-glucose; MM, minimal medium; AMM, Aspergillus MM. Shaking at 37°C was performed for all culture conditions.
b
Y, yes; N, no.