Fig 2.

Time course of TCE degradation and chloride production by PDN3 in chloride-free medium. Trichloroethylene was added at a concentration of 76.3 μM to 20 ml chloride-free medium in 125-ml Erlenmeyer flasks with screw caps and Teflon Mininert valves to prevent evaporation of TCE. The cultures were incubated at 150 rpm at 30°C. The filled diamonds indicate TCE removal by strain PDN3, the filled circles indicate TCE in the uninoculated control, the filled squares indicate chloride production from TCE by PDN3, the open circles indicate chloride in the uninoculated control with TCE, and the open squares indicate chloride in the uninoculated control without TCE.