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. 2012 May;78(9):3458–3464. doi: 10.1128/AEM.07985-11

Table 1.

Primers used in this work^a

Primer name	Sequence (5′→3′)
Primers for library construction
L101 M	`GGTATGGGATACGGAATGCTTTTGGCGGTTTGC`
D115G	`CAGGCTTTGTTTGACGGTTTATACCGTTACGTA`
L187I	`ACATTGATAAACAATATTTACAACCATTGTGTA`
Y224F	`GCATGGTACAAAGTGTTTGCTCAATATACAGG`
Y227F^b	`CTTGTGTCTCCTGTAAATTGAGCATACACTTTG`
G283P	`GATGCTACACGTTACCCGTGGAGAACTGCCGTG`
F293Y	`GTGGACTATTCATGGTATGGTGACCAGAGAGC`
I323L	`GTTGACGGATACACACTGCAAGGTTCAAAAATTAG`
Primers for mutagenesis
G177P+1	`GGTGCAATAAACTACCCGCAGGAAGCAAGGACATTG`
G177P+2	`CAATGTCCTTGCTTCCTGCGGGTAGTTTATTGCACC`
G373P+1	`GAATATTACGGATATTACCCGAACAGCTTGAGACTG`
G373P+2	`CAGTCTCAAGCTGTTCGGGTAATATCCGTAATATTC`
L101 M^b	`GCAAACCGCCAAAAGCATTCCGTATCCCATACC`
Y224F^b	`CCTGTATATTGAGCAAACACTTTGTACCATGC`
G283P^b	`CACGGCAGTTCTCCACGGGTAACGTGTAGCATC`

^a

Codon changes are underlined.

^b

Primer designed for complementary strand.