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Thermostability of the wild type and thermostable mutants
| Enzyme | Mutation | Tmapp (°C)a | ΔTm (°C) | Rate constant Kin (min−1)b |
|---|---|---|---|---|
| Cel8A | None | 80.7 | 1.558 | |
| G283P | G283P | 84.2 | 3.5 | 1.318 |
| TM | K276R, S329G, S375T | 86.9 | 6.2 | 0.239 |
| QM | K276R, G283P, S329G, S375T | 90.2 | 9.5 | 0.115 |
Tmapp is the approximate midpoint temperature of melting determined by CD spectroscopy at 222 nm.
The inactivation rate constants were deduced from plots of ln(% residual activity) versus time at 85°C.
