Table 2.
Basal and heat shock-induced expression of PdnaK-gfp in indicated backgrounds of E. coli
| Straina | RFUb,c noninduced | RFUb,c induced by heat shock | Ratio of parental strain vs HHP-resistant mutant | Ratio of induced vs noninduced strain |
|---|---|---|---|---|
| E. coli MG1655/pAA212 (parental strain) | 4.9 ± 0.2 | 28.1 ± 1.2 | 1.0 | 5.7 |
| E. coli DVL20/pAA212 (HHP-resistant mutant) | 7.5 ± 0.7 | 13.6 ± 0.5 | 1.5 | 1.8 |
| E. coli O157:H7/pAA212 (parental strain) | 3.6 ± 0.1 | 11.8 ± 0.5 | 1.0 | 3.3 |
| E. coli DVL24/pAA212 (HHP-resistant mutant) | 3.2 ± 0.2 | 10.3 ± 0.4 | 0.9 | 3.2 |
a
Please note that E. coli 536 and its HHP-resistant mutant (DVL25) were not included, as we were unable to transform them with plasmid pAA212.
b
RFU, relative fluorescence units.
c
All data shown are mean values ± standard deviations from an experiment with three independent replicates.