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Plasmids (pGETS109-pha series) used in this study. The phaA, phaB, and phaC genes are derived from R. eutropha. pha genes were inserted into the B. subtilis-E. coli shuttle vector pGETS109. Plasmids were constructed by the OGAB method (see Materials and Methods) with various pha gene orders (1 to 6).
