Table 2.
Biosynthesis of P(3HB) in recombinant E. coli DH5α harboring pGETS109-pha series plasmids and cultivated for 12 h or 72 ha
| Strain | Gene order | Culture time (h) | Dry cell wt (g/liter) | P(3HB) content (wt %) | Mol wt |
||
|---|---|---|---|---|---|---|---|
| Mn (×106) | Mw (×106) | Mw/Mn | |||||
| 1 | ABC | 12 | 1.6 ± 0.1 | 4.9 ± 0.4 | 3.3 ± 0.3 | 6.2 ± 0.4 | 1.9 |
| 72 | 8.0 ± 0.2 | 31 ± 1.0 | 3.8 ± 0.2 | 6.2 ± 0.1 | 1.7 | ||
| 2 | ACB | 12 | 1.3 ± 0.1 | 5.3 ± 0.7 | 1.5 ± 0.1 | 4.4 ± 0.3 | 2.9 |
| 72 | 8.6 ± 0.2 | 36 ± 2.4 | 1.4 ± 0.1 | 3.8 ± 0.4 | 2.7 | ||
| 3 | BAC | 12 | 1.4 ± 0.1 | 4.9 ± 0.2 | 2.2 ± 0.2 | 5.4 ± 0.2 | 2.5 |
| 72 | 9.4 ± 0.2 | 43 ± 0.9 | 1.6 ± 0.2 | 4.1 ± 0.4 | 2.7 | ||
| 4 | BCA | 12 | 1.5 ± 0.1 | 7.0 ± 0.9 | 2.8 ± 0.3 | 6.2 ± 0.1 | 2.5 |
| 72 | 9.3 ± 0.1 | 41 ± 1.4 | 2.3 ± 0.1 | 5.0 ± 0.1 | 2.2 | ||
| 5 | CAB | 12 | 1.7 ± 0.1 | 8.2 ± 0.9 | 1.6 ± 0.1 | 3.4 ± 0.3 | 3.1 |
| 72 | 10.8 ± 0.4 | 52 ± 3.3 | 0.8 ± 0.2 | 2.5 ± 0.4 | 3.3 | ||
| 6 | CBA | 12 | 2.6 ± 0.1 | 8.5 ± 1.0 | 1.5 ± 0.2 | 4.2 ± 0.2 | 2.8 |
| 72 | 11.2 ± 0.1 | 57 ± 1.6 | 0.5 ± 0.1 | 2.0 ± 0.1 | 3.8 | ||
a
Cells were cultivated in LB medium containing glucose (20 g/liter) at 30°C. The results are the averages from three independent experiments ± standard errors.