Fig 5.

Functional analysis of the surface-displayed β-agarases from Saccharophagus degradans 2-40. (A) TLC chromatogram of the reaction products on agaro-oligosaccharides (pATLIC-aga50A) or agarose (other displayed agarases). Lane S1, DP2 (neoagarobiose); lane S2, DP4 (neoagarotetraose) and DP6 (neoagarohexaose); lane 1, reaction products of pATLIC void vector; lane 2, pATLIC-aga50A; lane 3, pATLIC-aga16B; lane 4, pATLIC-aga86C; lane 5, pATLIC-aga50D; lane 6, pATLIC-aga86E. (B) The arrowheads in the SDS-PAGE indicate the overexpressed β-agarases fused to the translocator domain in the crude extract (lane M, molecular mass marker; other lane numbers are the same as for panel A).