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. 2012 May;78(9):3051–3058. doi: 10.1128/AEM.07004-11

Fig 6.

Fig 6

Controlled release of the displayed proteins by TEV protease. (A) Released Aga16B from the cells displaying Aga16B by TEV digestion. The relative activity of the amount of reducing sugars produced in whole-cell activity of untreated cells (UC), treated cells (TC), and the supernatant isolated from the cells displaying Aga16B after the TEV treatment (SU) is shown. (B) TLC analysis of the reaction mixtures for NABH-displaying cells and the released NABH. Lane 1, 3,6-anhydro-l-galactose (l-AHG); lane 2, d-galactose (d-Gal); lane 3, neoagarobiose (DP2); lane 4, reaction products of cells displaying pATLIC-NABH (no TEV protease treatment); lane 5, reaction products of cells displaying pATLIC-NABH (treated with TEV protease); lane 6, reaction products of the supernatant isolated from the cells displaying pATLIC-NABH after TEV protease pretreatment.